QIAprep Spin Miniprep Kit

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Manufacturer Prod Code: 27104

Pack : 50

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Citation Index: 21

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Description

For purification of up to 20 µg molecular biology grade plasmid DNA

  • Ready-to-use plasmid DNA in minutes
  • Reproducible yields of molecular biology grade plasmid DNA
  • Single protocol for high- and low-copy vectors
  • Even higher yields with the High-Yield Supplementary Protocol
  • Improved QIAprep 2.0 Spin Column
  • GelPilot loading dye for convenient sample analysis
The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 µg high-purity plasmid or cosmid DNA for use in routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. Even higher yields (up to 30 µg) can be achieved using the High-Yield Supplementary Protocol. The QIAprep Spin Miniprep Kit can be automated on the QIAcube. For optimal results it is recommended to use this product together with QIAvac 24 Plus.

The QIAprep procedure.

Complete digestion with various restriction enzymes.
Restriction analysis of pBluescript DNA purified with the QIAprep Spin Miniprep Kit is shown. Digestion with the indicated enzymes (1–5 units) was carried out on 1 µg plasmid DNA. Markers: Lambda-HindIII.
GelPilot Loading Dye
The 3 tracking dyes enable easy optimization of gel electrophoresis times and monitoring of small DNA fragments.
Spin column handling options — B
Spin column handling options — A.
Microcentrifuge.
Spin column handling options — C.
QIAcube.

The QIAprep procedure.

Performance

The QIAprep Spin Miniprep Kit enables purification of up to 20 µg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep 2.0 Spin Columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube (see figures "QIAprep 2.0 Spin Column handling options , and "). The vacuum procedure provides simplified handling and faster sample processing. QIAprep 2.0 Spin Columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.

 
QIAprep Spin Miniprep Kit specifications
Format Spin columns
Purification module QIAprep 2.0 Spin Columns
Throughput 1–24 samples
Preparation time 24 minipreps in 30 minutes
Equipment required Microcentrifuge or vacuum manifold; fully automatable using the QIAcube
Lysate clearing Centrifugation
Capacity of column reservoir 800 µl
Minimum elution buffer volume 50 µl
Culture volume for high-copy plasmids 1–5 ml
Culture volume for low-copy plasmids/cosmids 1–10 ml

Purified DNA can be used in restriction digestion (see figure ""). 

Principle
QIAprep 2.0 Spin Columns contain a unique silica membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenolchloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep 2.0 Spin Columns is immediately ready to use – there is no need to precipitate, concentrate, or desalt. To enable faster and more convenient sample processing and analysis, gel loading dye is provided in the kit. GelPilot Loading Dye contains 3 tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "").
Procedure
Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure (see flowchart ""). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep 2.0 module where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water. In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.
Applications
The QIAprep Miniprep Kits provides reproducible yields of high-purity DNA suitable for use in most applications, including:
  • PCR
  • Restriction digestion
  • Ligation and transformation
  • Sequencing
  • Screening

Manufacturer Prod Code: 27104

Pack : 50

Sold By:

Citation Index: 21



Labviva Id: LV-0009-3388

Specifications

More Information
Attribute Type Attribute Value
Manufacturer QIAGEN
Detection Method Spin Column
Detection Method Spin Column
Quantity 50
Sample Type Agarose Gels, Bacteria, Biofilms, Blood, Buccal Brush, Cells, Cultured cells, E.Coli, Food, Formalin fixed paraffin embedded tissue samples, Fungal Cell Culture, Liquid, Mouse tail, Plant Samples, Plasma, Purified DNA, RNA Preps, Seeds, Serum, Soil, Stool
Species Bacteria
Processing Manual (vacuum or centrifugation)
Culture volume/starting material 1–10 ml culture volume
Elution volume 50 µl (minimal)
Plasmid type High-copy, low-copy, cosmid DNA
Samples per run; throughput 1–24 samples per run
Time per run or prep per run <30 minutes
Yield <20 µg

Protocols

Chromatin Immunoprecipitation (ChIP) Assay for Detecting Direct and Indirect Protein – DNA Interactions in Magnaporthe oryzae

Gang Li,Margarita Marroquin-Guzman,Richard A. Wilson

Vol 5, Iss 21, November 05, 2015

| Source:

An Affinity-directed Protein Missile (AdPROM) System for Targeted Destruction of Endogenous Proteins

Gopal P Sapkota,Luke J Fulcher,Thomas J Macartney

Vol 7, Iss 22, November 20, 2017

| Source:

Assaying the Effects of Splice Site Variants by Exon Trapping in a Mammalian Cell Line

Stuart W. Tompson,Terri L. Young

Vol 7, Iss 10, May 20, 2017

| Source:

Transfer of Large Contiguous DNA Fragments onto a Low Copy Plasmid or into the Bacterial Chromosome

Analise Z Reeves,Cammie F Lesser

Vol 6, Iss 22, November 20, 2016

| Source:

Telomere-mediated Chromosomal Truncation via Agrobacterium tumefaciens or Particle Bombardment to Produce Engineered Minichromosomes in Plants

Changzeng Zhao,James A. Birchler,Jon P. Cody,Nathan C. Swyers*,Nathaniel D. Graham*,Robert T. Gaeta

Vol 5, Iss 18, September 20, 2015

| Source:

Dense sgRNA Library Construction Using a Molecular Chipper Approach

Jijun Cheng,Jun Lu,Wen Pan

Vol 7, Iss 12, June 20, 2017

| Source:

References

Chimeric influenza haemagglutinins: Generation and use in pseudotype neutralization assays

Francesca Ferrara; Nigel Temperton

MethodsX; Volume 4, 2017, Pages 11-24

View Reference

Characterisation of novel biomass degradation enzymes from the genome of Cellulomonas fimi

Steven D. Kane; Christopher E. French

Enzyme and Microbial Technology; Volume 113, June 2018, Pages 9-17

View Reference

Asymmetry between Activation and Deactivation during a Transcriptional Pulse

Lee S.S. Dunham; Hiroshi Momiji; Claire V. Harper; Polly J. Downton; Kirsty Hey; Anne McNamara; Karen Featherstone; David G. Spiller; David A. Rand; Bärbel Finkenstädt; Michael R.H. White; Julian R.E. Davis

Cell Systems; Volume 5, Issue 6 2017/12/27

View Reference

Description and plasmid characterization of qnrD determinants in Proteus mirabilis and Morganella morganii

A. Mazzariol; B. Kocsis; R. Koncan; E. Kocsis; P. Lanzafame; G. Cornaglia

Clinical Microbiology and Infection; Volume 18, Issue 3 2012/03/01

View Reference

Comparison of LightCycler PCR and culture for detection of group B streptococci from vaginal swabs

M. Convert; G. Martinetti Lucchini; M. Dolina; J.-C. Piffaretti

Clinical Microbiology and Infection; Volume 11, Issue 12 2005/12/01

View Reference

A highly sensitive quantitative real-time PCR assay based on the groEL gene of contemporary Thai strains of Orientia tsutsugamushi

D.H. Paris; N. Aukkanit; K. Jenjaroen; S.D. Blacksell; N.P.J. Day

Clinical Microbiology and Infection; Volume 15, Issue 5 2009/05/01

View Reference

Genetic characterization of HIV-1 from semen and blood from clade C-infected subjects from India and effect of therapy in these body compartments

Chengli Shen; Ming Ding; Jodi K. Craigo; Patrick Tarwater; Ramdas Chatterjee; Pratima Roy; Subhasish K. Guha; Bibhuti Saha; Dolonchapa Modak; Dhrubak Neogi; Yue Chen; Phalguni Gupta

Virology; Volume 401, Issue 2 2010/06/05

View Reference

Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac2SGL

Frank Camacho; María E. Sarmiento; Fatima Reyes; Louise Kim; Jim Huggett; Marco Lepore; Oscar Otero; Martine Gilleron; Germain Puzo; Mohd Nor Norazmi; Graham Rook; Lucia Mori; Gennaro De Libero; Armando Acosta

International Journal of Mycobacteriology; Volume 5, Issue 2, June 2016, Pages 120-127

View Reference

IncX3 plasmid mediated occurrence of blaNDM-4 within Escherichia coli ST448 from India

Nargis A. Choudhury; Deepjyoti Paul; Atanu Chakravarty; Amitabha Bhattacharjee; Debadatta Dhar Chanda

Journal of Infection and Public Health; Volume 11, Issue 1, January–February 2018, Pages 111-114

View Reference

Characterisation of Major Histocompatibility Complex Class I in the Australian Cane Toad, Rhinella marina

Mette Lillie, Richard Shine, Katherine Belov

PLOS ONE; Published: August 5, 2014

View Reference

A Robust and Efficient Production and Purification Procedure of Recombinant Alzheimers Disease Methionine-Modified Amyloid-ß Peptides

Marie Hoarau, Yannick Malbert , Romain Irague , Christelle Hureau , Peter Faller , Emmanuel Gras , Isabelle André , Magali Remaud-Siméon

PLOS ONE; Published: August 17, 2016

View Reference

A Robust and Efficient Production and Purification Procedure of Recombinant Alzheimers Disease Methionine-Modified Amyloid-ß Peptides

Marie Hoarau, Yannick Malbert , Romain Irague , Christelle Hureau , Peter Faller , Emmanuel Gras , Isabelle André , Magali Remaud-Siméon

PLOS ONE; Published: August 17, 2016

View Reference

Characterisation of Major Histocompatibility Complex Class I in the Australian Cane Toad, Rhinella marina

Mette Lillie, Richard Shine, Katherine Belov

PLOS ONE; Published: August 5, 2014

View Reference

Selection of phage-displayed human antibody fragments specific for CD1b presenting the Mycobacterium tuberculosis glycolipid Ac2SGL

Frank Camacho; María E. Sarmiento; Fatima Reyes; Louise Kim; Jim Huggett; Marco Lepore; Oscar Otero; Martine Gilleron; Germain Puzo; Mohd Nor Norazmi; Graham Rook; Lucia Mori; Gennaro De Libero; Armando Acosta

International Journal of Mycobacteriology;

View Reference

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