Alkaline Phosphatase Detection Kit

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.

Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.


Embryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore's proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.

Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].

For Research Use Only; Not for use in diagnostic procedures

Research Category
Stem Cell Research

General description

Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

Storage and Stability

The Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.

When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.

Labviva Id: LV-0507-5418



More Information
Attribute Type Attribute Value
Species Reactivity MAMMALIAN
Brand Family Chemicon
References Count 10.0000
Applications Stem Cell Culture
Stem Cell Type Mouse Embryonic Stem Cells, Human Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells, Hematopoietic Stem Cells, Epithelial Cells, Pancreatic Stem Cells, Cardiac Stem Cells, Induced Pluripotent Stem Cells
Kit Assay Type Stem Cell Kit
Packaging 100 assays (SCR004)
Thomas Number C943U48
Trade Name Chemicon
Shipped In wet ice

Protocols & Papers

Protocols & Papers

Efficient iPS cell production with the MyoD transactivation domain in serum-free culture.

Hiroyuki Hirai,Nobuko Katoku-Kikyo,Peter Karian,Meri Firpo,Nobuaki Kikyo

PloS one; 7

View Reference

BMP4 Signaling Acts via dual-specificity phosphatase 9 to control ERK activity in mouse embryonic stem cells.

Zhongwei Li,Teng Fei,Jianping Zhang,Gaoyang Zhu,Lu Wang,Danyu Lu,Xiaochun Chi,Yan Teng,Ning Hou,Xiao Yang,Hongquan Zhang,Jing-Dong J Han,Ye-Guang Chen

Cell stem cell; 10

View Reference

Efficient feeder-free episomal reprogramming with small molecules.

Junying Yu,Kevin Fongching Chau,Maxim A Vodyanik,Jinlan Jiang,Yong Jiang

PloS one; 6

View Reference

Large scale phosphoproteome profiles comprehensive features of mouse embryonic stem cells.

Li, QR; Xing, XB; Chen, TT; Li, RX; Dai, J; Sheng, QH; Xin, SM; Zhu, LL; Jin, Y; Pei, G; Kang, JH; Li, YX; Zeng, R

Molecular & cellular proteomics : MCP; 10

View Reference

Epigenetic regulation of Nanog expression by Ezh2 in pluripotent stem cells.

Villasante, A; Piazzolla, D; Li, H; Gomez-Lopez, G; Djabali, M; Serrano, M

Cell cycle (Georgetown Tex.); 10

View Reference

Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.

Zhao Cheng,Sachiko Ito,Naomi Nishio,Hengyi Xiao,Rong Zhang,Haruhiko Suzuki,Yayoi Okawa,Toyoaki Murohara,Ken-Ichi Isobe

Journal of molecular cell biology; 3

View Reference

Ethanol, acetaldehyde, and estradiol affect growth and differentiation of rhesus monkey embryonic stem cells.

Catherine A VandeVoort,Dana L Hill,Charles L Chaffin,Alan J Conley

Alcoholism clinical and experimental research; 35

View Reference

Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.

Derk ten Berge,Dorota Kurek,Tim Blauwkamp,Wouter Koole,Alex Maas,Elif Eroglu,Ronald K Siu,Roel Nusse

Nature cell biology; 13

View Reference

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