Alkaline Phosphatase Detection Kit
Description
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components
Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.
Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.
Application
Embryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore's proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.
Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].
For Research Use Only; Not for use in diagnostic procedures
Research Category
Stem Cell Research
General description
Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
Storage and Stability
The Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.
When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
Labviva Id: LV-0507-5418
Specifications
Attribute Type | Attribute Value |
Species Reactivity | MAMMALIAN |
Brand Family | Chemicon |
References Count | 10.0000 |
Applications | Stem Cell Culture |
Stem Cell Type | Mouse Embryonic Stem Cells, Human Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells, Hematopoietic Stem Cells, Epithelial Cells, Pancreatic Stem Cells, Cardiac Stem Cells, Induced Pluripotent Stem Cells |
Kit Assay Type | Stem Cell Kit |
Manufacturer | MILLIPORE SIGMA |
Packaging | 100 assays (SCR004) |
Thomas Number | C943U48 |
Trade Name | Chemicon |
Shipped In | wet ice |
Protocols & Papers

Efficient iPS cell production with the MyoD transactivation domain in serum-free culture.
Hiroyuki Hirai,Nobuko Katoku-Kikyo,Peter Karian,Meri Firpo,Nobuaki Kikyo
PloS one; 7

Establishment of LIF-dependent human iPS cells closely related to basic FGF-dependent authentic iPS cells.
Hiroyuki Hirai,Meri Firpo,Nobuaki Kikyo
PloS one; 7

BMP4 Signaling Acts via dual-specificity phosphatase 9 to control ERK activity in mouse embryonic stem cells.
Zhongwei Li,Teng Fei,Jianping Zhang,Gaoyang Zhu,Lu Wang,Danyu Lu,Xiaochun Chi,Yan Teng,Ning Hou,Xiao Yang,Hongquan Zhang,Jing-Dong J Han,Ye-Guang Chen
Cell stem cell; 10

Efficient feeder-free episomal reprogramming with small molecules.
Junying Yu,Kevin Fongching Chau,Maxim A Vodyanik,Jinlan Jiang,Yong Jiang
PloS one; 6
Large scale phosphoproteome profiles comprehensive features of mouse embryonic stem cells.
Li, QR; Xing, XB; Chen, TT; Li, RX; Dai, J; Sheng, QH; Xin, SM; Zhu, LL; Jin, Y; Pei, G; Kang, JH; Li, YX; Zeng, R
Molecular & cellular proteomics : MCP; 10
Epigenetic regulation of Nanog expression by Ezh2 in pluripotent stem cells.
Villasante, A; Piazzolla, D; Li, H; Gomez-Lopez, G; Djabali, M; Serrano, M
Cell cycle (Georgetown Tex.); 10
Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.
Zhao Cheng,Sachiko Ito,Naomi Nishio,Hengyi Xiao,Rong Zhang,Haruhiko Suzuki,Yayoi Okawa,Toyoaki Murohara,Ken-Ichi Isobe
Journal of molecular cell biology; 3
Ethanol, acetaldehyde, and estradiol affect growth and differentiation of rhesus monkey embryonic stem cells.
Catherine A VandeVoort,Dana L Hill,Charles L Chaffin,Alan J Conley
Alcoholism clinical and experimental research; 35
Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.
Derk ten Berge,Dorota Kurek,Tim Blauwkamp,Wouter Koole,Alex Maas,Elif Eroglu,Ronald K Siu,Roel Nusse
Nature cell biology; 13
Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD.
Hirai, Hiroyuki, et al.
Stem Cells 29: 1349-61 (2011); 2011