GenUP™ Exo SAP Kit

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Manufacturer Prod Code: BR0701803

Pack : 2000

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Description

Features

  • Fast 15 min PCR cleanup without the need for spin columns
  • 100% recovery — no loss of PCR product
  • Simple process — just add Exonuclease 1 and Shrimp Alkaline Phosphatase to PCR mix
  • Easy scalability and automation

Applications

  • PCR cleanup prior to sequencing or genotyping
  • Removal of nucleotides and primers from PCR reaction
biotechrabbit™ GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Exonuclease 1 (Exo) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products.Both enzymes are active in PCR buffers and completely inactivated by heating for 10 min at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 5' ? 3' exonuclease activity of Exonuclease 1 degrades single-stranded primers.

 

Component

Composition

Shrimp Alkaline Phosphatase

Shrimp Alkaline Phosphatase in storage buffer containing 50% glycerol (v/v).

Exonuclease 1

Exonuclease 1 in storage buffer containing 50% glycerol (v/v).

 

STORAGE

-20°C (until expiry date – see product label)

 

Quality Control
Shrimp Alkaline Phosphatase
Specific activity

One unit Shrimp Alkaline Phosphatase, 1 U/µl, converts 1 µmol of p-nitrophenyl phosphate per minute to nitrophenol and phosphate at 37°C and pH 10.4 in 0.1 M glycine buffer, 1 mM ZnCl2, 1 mM MgCl2 and
6 mM 4-nitrophenyl phosphate.

Purity

Purified to apparent homogeneity by SDS-PAGE. DNase, RNase and protease activities were not detected. Shrimp Alkaline Phosphatase is highly pure and is tested free of contaminating nucleases.

Exonuclease 1
Specific activity

Exonuclease 1 is tested for exonuclease activity with a specific activity of > 130,000 U/mg.

Purity

No endonuclease contamination was detected DNA was incubated with 200 U Exonuclease 1 for 4 h at 37 °C.

 

STEPS

  • Add Shrimp Alkaline Phosphatase and Exonuclease 1 to a completed PCR reaction.
  • Incubate at 37°C for 5 min.
  • Heat inactivate at 80°C for 10 min.

For detailed protocol, please, refer to the product manual (Related Documents).

Manufacturer Prod Code: BR0701803

Pack : 2000

Sold By:



Labviva Id: LV-0009-3649

Specifications

More Information
Attribute Type Attribute Value
Manufacturer BIOTECH RABBIT
Detection Method Shrimp Alkaline Phospatase
Size 2000 rxn
Quantity 2000
Sample Type Agarose Gels, Bacteria, Biofilms, Blood, Buccal Brush, Cells, Cultured cells, E.Coli, Food, Formalin fixed paraffin embedded tissue samples, Fungal Cell Culture, Liquid, Mouse tail, Plant Samples, Plasma, Purified DNA, RNA Preps, Seeds, Serum, Soil, Stool
Species Bacteria, Bovine, Eukaryotes, Fungi, Human, Mammilian, Plant, Rodent
Contents 4 × 500 µl Shrimp Alkaline Phosphatase
4 × 500 µl Exonuclease 1

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