GenUP™ Exo SAP Kit

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Manufacturer Prod Code: BR0701803

Pack : 2000

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Description

Features

  • Fast 15 min PCR cleanup without the need for spin columns
  • 100% recovery — no loss of PCR product
  • Simple process — just add Exonuclease 1 and Shrimp Alkaline Phosphatase to PCR mix
  • Easy scalability and automation

Applications

  • PCR cleanup prior to sequencing or genotyping
  • Removal of nucleotides and primers from PCR reaction

biotechrabbit™ GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.

The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Heat Labile Exonuclease 1 (HL-ExoI) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products.

Both enzymes are active in PCR buffers and completely inactivated by heating at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 5' ? 3' exonuclease activity of Exonuclease 1 degrades single-stranded primers.

Other Information

 

Component

Composition

Shrimp Alkaline Phosphatase

2 U/µl SAP in storage buffer containing 50% glycerol (v/v).

Heat Labile Exonuclease 1

10 U/µl HL-ExoI in storage buffer containing 50% glycerol (v/v).

 

STORAGE

-20°C (until expiry date – see product label)

 

Quality Control
Shrimp Alkaline Phosphatase
Unit definition

One unit Shrimp Alkaline Phosphatase, 1 U/µl, converts 1 µmol of p-nitrophenyl phosphate per minute to nitrophenol and phosphate at 37°C and pH 10.4 in 0.1 M glycine buffer, 1 mM ZnCl2, 1 mM MgCl2 and 6 mM 4-nitrophenyl phosphate.

Specificity

SAP Removes 5’-phosphates from DNA, RNA, dNTPs and proteins.

Purity

Purified to apparent homogeneity. SAP is highly pure and tested free of contaminating nucleases.

Heat Labile Exonuclease 1
Unit definition

One unit catalyses the release of 10 nmol of acid soluble nucleotides in 30 min at 25°C in 20 mM Tris-HCl pH 7.5, 200 mM NaCl, 20 mM MgCl2, 1 mM DTT, 0.01% Triton X-100, 2.5% glycerol, 3H-dATP-labelled DNA (25 ng/µl).

Specificity

HL-ExoI is a 3’-5’ exonuclease, specific for single stranded DNA.

Purity

Purified to apparent homogeneity. HL-ExoI is highly pure and tested free of contaminating nucleases.

Protocol

 

STEPS

  • Add 1 µl SAP (2 U) and 1 µl HL-ExoI (10 U) to 5 µl of a completed PCR reaction.
  • Incubate at 37°C for 5 min.
  • Heat inactivate at 80°C for 10 min.

For detailed protocol, please, refer to the product manual (Related Documents).

Manufacturer Prod Code: BR0701803

Pack : 2000

Sold By:



Labviva Id: LV-0009-3649

Specifications

More Information
Attribute Type Attribute Value
Quantity 2000
Contents 4 kits BR0701802 GenUP™ Exo SAP Kit (500 rxn each)
Manufacturer BIOTECH RABBIT
Size 2000 rxn
Detection Method Shrimp Alkaline Phospatase
Sample Type Agarose Gels, Bacteria, Biofilms, Blood, Buccal Brush, Cells, Cultured cells, E.Coli, Food, Formalin fixed paraffin embedded tissue samples, Fungal Cell Culture, Liquid, Mouse tail, Plant Samples, Plasma, Purified DNA, RNA Preps, Seeds, Serum, Soil, Stool
Species Bacteria, Bovine, Eukaryotes, Fungi, Human, Mammilian, Plant, Rodent

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