proBDNF Rapid ELISA Kit: Human, Mouse, Rat (2 Plates)

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Description

Description

Try our new proBDNF Rapid ELISA kit (BEK-2237-2P) for increased sensitivity in human blood samples!

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The Biosensis proBDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, cell lysates, serum, citrate-plasma and tissue extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples.

This ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance.

This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins.

Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples.

Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately.

This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.


Labviva Id: LV-0358-0454

Specifications

Specifications

More Information
Attribute Type Attribute Value
Species Reactivity HUMAN, RODENT
Antibody Isotype Monoclonal-Polyclonal
Application Details Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Name Brain-derived neurotrophic factor; BDNF; Abrineurin
Target Protein BDNF
References Count 4.0000
Uniprot Number Only P23560
Uniprot Full Accession Number & Name P23560 BDNF_HUMAN;
Storage Store at 4 °C
Product Specificity Human proBDNF. The capture antibody used in this ELISA kit binds to epitopes within the pro-domain of proBDNF. Thus, this ELISA detects the full length and potentially truncated form of proBDNF and does not quantify mature BDNF.
A cross-reactivity of 2%
UNSPSC Code 41116158
Shipping Temperature 2-8°C (on cold pack)
Expiry Date 12 months from purchase.
Applications ELISA
Manufacturer BIOSENSIS
Sensitivity Typical limit of detection (LOD) for proBDNF is 10 pg/mL determined as 150% of the blank value.
Related Products BL-004-500, Heterophilic antibody blocker: Sample diluent additive for accurate quantification of proBDNF in human serum and citrate-plasma.

BEK-2240: Mature BDNF/proBDNF Combo Rapid ELISA Kit
Immunogen Recombinant human proBDNF, mutated to be cleavage-resistant, made in 293F cells
Product Type ELISA
Format complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions., Ready to use Assay
Product Size 2 plates
Background Info BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia
Kit Components The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Target Cross Reactivity A cross-reactivity of 2% in weight concentRation (0.9% in molar concentRation) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.
Due to a high degree of sequence homology, this Human proBDNF ELISA kit cross-reacts w
Range 15.6 - 1000 pg/mL

Supporting Literature

Protocols & Papers

Protocols & Papers

BDNF Val66Met and childhood adversity on response to physical exercise and internet-based cognitive behavioural therapy in depressed Swedish adults.

Rahman MS; Millischer V; Zeebari Z; Forsell Y; Lavebratt C

Journal of psychiatric research.; Volume 93, Issue 2017/10/01

View Reference

Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures.

Riffault B; Kourdougli N; Dumon C; Ferrand N; Buhler E; Schaller F; Chambon C; Rivera C; Gaiarsa JL; Porcher C

Cerebral cortex (New York, N.Y. : 1991).; Volume 28, Issue 2 2018/02/01

View Reference

Pro-brain-derived neurotrophic factor inhibits GABAergic neurotransmission by activating endocytosis and repression of GABAA receptors.

Riffault B; Medina I; Dumon C; Thalman C; Ferrand N; Friedel P; Gaiarsa JL; Porcher C

The Journal of neuroscience : the official journal of the Society for Neuroscience.; Volume 34, Issue 40 2014/10/01

View Reference

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