proBDNF Rapid ELISA Kit: Human, Mouse, Rat (1 Plate)
This ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance.
This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins.
Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples.
Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for .
This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Labviva Id: LV-0358-0463
|Attribute Type||Attribute Value|
|Species Reactivity||HUMAN, RODENT|
|Application Details||Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.|
|Alternative Name||Brain-derived neurotrophic factor; BDNF; Abrineurin|
|Uniprot Number Only||P23560|
|Uniprot Full Accession Number & Name||P23560 BDNF_HUMAN;|
|Storage||Store at 4 °C|
Human proBDNF. The capture antibody used in this ELISA kit binds to epitopes within the pro-domain of proBDNF. Thus, this ELISA detects the full length and potentially truncated form of proBDNF and does not quantify mature BDNF.
A cross-reactivity of 2%
|Shipping Temperature||2-8°C (on cold pack)|
|Expiry Date||12 months from purchase.|
|Sensitivity||Typical limit of detection (LOD) for proBDNF is 10 pg/mL determined as 150% of the blank value.|
BL-004-500, Heterophilic antibody blocker: Sample diluent additive for accurate quantification of proBDNF in human serum and citrate-plasma.
BEK-2240: Mature BDNF/proBDNF Combo Rapid ELISA Kit
|Immunogen||Recombinant human proBDNF, mutated to be cleavage-resistant, made in 293F cells|
|Format||complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions., Ready to use Assay|
|Product Size||1 plate|
|Background Info||BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia|
|Kit Components||The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.|
|Target Cross Reactivity||
A cross-reactivity of 2% in weight concentRation (0.9% in molar concentRation) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.
Due to a high degree of sequence homology, this Human proBDNF ELISA kit cross-reacts w
|Range||15.6 - 1000 pg/mL|
Protocols & Papers
BDNF Val66Met and childhood adversity on response to physical exercise and internet-based cognitive behavioural therapy in depressed Swedish adults.
Rahman MS; Millischer V; Zeebari Z; Forsell Y; Lavebratt C
Journal of psychiatric research.; Volume 93, Issue 2017/10/01
Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures.
Riffault B; Kourdougli N; Dumon C; Ferrand N; Buhler E; Schaller F; Chambon C; Rivera C; Gaiarsa JL; Porcher C
Cerebral cortex (New York, N.Y. : 1991).; Volume 28, Issue 2 2018/02/01
Astrocytes Protect against Isoflurane Neurotoxicity by Buffering pro-brain-derived Neurotrophic Factor.
Stary CM; Sun X; Giffard RG
Anesthesiology.; Volume 123, Issue 4 2015/10/01
Pro-brain-derived neurotrophic factor inhibits GABAergic neurotransmission by activating endocytosis and repression of GABAA receptors.
Riffault B; Medina I; Dumon C; Thalman C; Ferrand N; Friedel P; Gaiarsa JL; Porcher C
The Journal of neuroscience : the official journal of the Society for Neuroscience.; Volume 34, Issue 40 2014/10/01