Oligomeric Amyloid-beta ELISA Kit (1 plate)
Description
The oligomeric form of Amyloid Beta peptide (Aß, 1-42) has been closely linked to Alzheimer€™s Disease. Several ELISAs targeting Aß have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al., 2012), has been shown to specifically detect Aß, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of Aß peptide (o-Aß) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody.The Biosensis oligomeric Aß ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric Aß peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-Aß capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-Aß present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric Aß peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-Aß standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit.
This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Labviva Id: LV-0358-0812
Specifications
| Attribute Type | Attribute Value |
| Species Reactivity | RODENT |
| Antibody Isotype | Monoclonal-Monoclonal |
| Application Details | Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed. |
| Alternative Name | Beta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein; MOAB2; MOAB-2; Alzheimer's antibody; AB40; AB42; abeta. |
| Target Protein | A4 |
| References Count | 6.0000 |
| Uniprot Number Only | P05067 |
| Uniprot Full Accession Number & Name | P05067 |
| Storage | Store at 4 °C |
| Product Specificity | Human. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aß residues 1-4 and is highly specific just to amyloid beta peptide.The Biosensis o-Aß Elisa detects Aß oligomers as validated and described by Youmans KL et al (2012) and Rat by Combes |
| UNSPSC Code | 41116158 |
| Shipping Temperature | 2-8°C (on cold pack) |
| Expiry Date | 12 months from purchase. |
| Applications | ELISA |
| Manufacturer | BIOSENSIS |
| Related Products |
Oligomeric Human beta-Amyloid Aß1-42 Peptide, Stabilized (PE-1750-1000) Beta-Amyloid Aß1-42 Peptide, HFIP-treated (PE-1749-50) |
| Immunogen | The standard in this ELISA is synthetically manufactured beta-amyloid peptide, amino acids 1-42 of human, HFIP treated and dried.The stabilized oligomeric beta amyloid 1-42 control complex is also constructed from the same synthetic peptide standard material. No animal systems were used for their manufacture. |
| Product Type | ELISA |
| Format | complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions., Ready to use Assay |
| Product Size | 1 plate |
| Kit Components | The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols. |
| Target Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
Protocols & Papers
Targeting Apolipoprotein E/Amyloid ß Binding by Peptoid CPO_Aß17-21?P Ameliorates Alzheimer's Disease Related Pathology and Cognitive Decline.
Liu S; Park S; Allington G; Prelli F; Sun Y; Martá-Ariza M; Scholtzova H; Biswas G; Brown B; Verghese PB; Mehta PD; Kwon YU; Wisniewski T
Scientific reports.; Volume 7, Issue 1 2017/08/14
Particulate air pollutants, APOE alleles and their contributions to cognitive impairment in older women and to amyloidogenesis in experimental models.
Cacciottolo M; Wang X; Driscoll I; Woodward N; Saffari A; Reyes J; Serre ML; Vizuete W; Sioutas C; Morgan TE; Gatz M; Chui HC; Shumaker SA; Resnick SM; Espeland MA; Finch CE; Chen JC
Translational psychiatry.; Volume 7, Issue 1 2017/01/31
Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice.
Thomas R; Zuchowska P; Morris AW; Marottoli FM; Sunny S; Deaton R; Gann PH; Tai LM
Acta neuropathologica communications.; Volume 4, Issue 1 2016/10/27
Tocotrienol-Rich Fraction Modulates Amyloid Pathology and Improves Cognitive Function in AßPP/PS1 Mice.
Ibrahim NF; Yanagisawa D; Durani LW; Hamezah HS; Damanhuri HA; Wan Ngah WZ; Tsuji M; Kiuchi Y; Ono K; Tooyama I
Journal of Alzheimer's disease : JAD.; Volume 55, Issue 2 2017
A multifunctional therapeutic approach to disease modification in multiple familial mouse models and a novel sporadic model of Alzheimer's disease.
Luo J; Lee SH; VandeVrede L; Qin Z; Ben Aissa M; Larson J; Teich AF; Arancio O; D'Souza Y; Elharram A; Koster K; Tai LM; LaDu MJ; Bennett BM; Thatcher GR
Molecular neurodegeneration.; Volume 11, Issue 2016/04/29
Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease.
Peng W; Achariyar TM; Li B; Liao Y; Mestre H; Hitomi E; Regan S; Kasper T; Peng S; Ding F; Benveniste H; Nedergaard M; Deane R
Neurobiology of disease.; Volume 93, Issue 2016/09/01
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