Mature BDNF Rapid ELISA Kit: Human, Mouse, Rat (1 plate)

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BEK-2211-1P
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Description

Description

The Biosensis Mature BDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mature BDNF in less than 3 hours in cell culture supernatants, serum, plasma (citrate and EDTA), cell lysates, human milk and brain extracts only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Note that accurate quantification of BDNF in human milk requires a secretory IgA (sIgA) blocker which can be purchased separately (BL-001-1250). For measurement of mature BDNF in CSF samples, please contact us at sales@biosensis.com.

This ELISA kit has been tested in independent research laboratories and found to achieve highest reproducibility with intra- and inter-assay CVs as low as 1% and 5%, respectively (Polacchini et al., 2015).

This ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3€™,5,5€™-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF present in samples and protein standards. A BDNF positive control (QC sample) is provided to assure consistent assay performance.

This Mature BDNF ELISA kit employs a recombinant human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen.

This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures. For in-vitro diagnostic (IVD) applications in the European Economic Area (EEA), we refer to the CE Marked BDNF
RapidTM ELISA kit (BEK-2211-CE).


Labviva Id: LV-0358-0022

Specifications

Specifications

More Information
Attribute Type Attribute Value
Product Size 1 plate
Expiry Date 12 months from purchase.
Shipping Temperature 2-8°C (on cold pack)
Unspsc Code 41116158
Product Specificity Human, mouse, rat and numerous other species. | No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25
Uniprot Number only P23560
Uniprot Full Accession Number & Name P23560 BDNF_HUMAN;
Alternative Name Brain-derived neurotrophic factor; BDNF; Abrineurin
Application Details Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Antibody Isotype Monoclonal-Monoclonal
Immunogen Recombinant human BDNF with an N-terminal methionine residue, made in E. coli (WHO reference reagent)
Related Products BL-001-1250, Secretory IgA (sIgA) blocker: Sample diluent additive for accurate quantification or BDNF in human milk.

BEK-2237-1P: Human proBDNF Rapid ELISA Kit

BEK-2240: Mature BDNF/proBDNF Combo Rapid ELISA Kit
Format complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions., Ready to use Assay
Applications ELISA
product type ELISA
Species reactivity HUMAN, RODENT
Storage Store at 4 °C
References Count 12.0000
Target Protein BDNF
Manufacturer BIOSENSIS
PRODUCTNAME Mature BDNF Rapid ELISA Kit: Human, Mouse, Rat (1 plate)
Background Info BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia
Kit Components The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Target Cross Reactivity No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-le
range 7.8 pg/mL - 500 pg/mL
Sensitivity Typical limit of detection (LOD) for BDNF is less than 2 pg/mL, determined as 150% of the blank value.

Supporting Literature

Protocols & Papers

Protocols & Papers

Evaluation of plasma brain-derived neurotrophic factor levels and self-perceived cognitive impairment post-chemotherapy: a longitudinal study.

Ng T; Lee YY; Chae JW; Yeo AHL; Shwe M; Gan YX; Ng RCH; Chu PPY; Khor CC; Ho HK; Chan A

BMC cancer.; Volume 17, Issue 1 2017/12/19

View Reference

Increased serum levels of mBDNF in women with minimal and mild endometriosis have no predictive power for the disease.

Perricos A; Ashjaei K; Husslein H; Proestling K; Kuessel L; Obwegeser R; Wenzl R; Yotova I

Experimental biology and medicine (Maywood, N.J.).; Volume 243, Issue 1 2018/01/01

View Reference

Effects of long-term exercise and low-level inhibition of GABAergic synapses on motor control and the expression of BDNF in the motor related cortex.

Inoue T; Ninuma S; Hayashi M; Okuda A; Asaka T; Maejima H

Neurological research.; Volume 40, Issue 1 2018/01/01

View Reference

Brain-derived neurotrophic factor in adjuvant-induced arthritis in rats. Relationship with inflammation and endothelial dysfunction.

Pedard M; Demougeot C; Prati C; Marie C

Progress in neuro-psychopharmacology & biological psychiatry.; Volume 82, Issue 2018/03/02

View Reference

Epigenetic Alterations Affecting Transcription Factors and Signaling Pathways in Stromal Cells of Endometriosis.

Yotova I; Hsu E; Do C; Gaba A; Sczabolcs M; Dekan S; Kenner L; Wenzl R; Tycko B

PloS one.; Volume 12, Issue 1 2017/01/26

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Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures.

Riffault B; Kourdougli N; Dumon C; Ferrand N; Buhler E; Schaller F; Chambon C; Rivera C; Gaiarsa JL; Porcher C

Cerebral cortex (New York, N.Y. : 1991).; Volume 28, Issue 2 2018/02/01

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Remote Ischemic Preconditioning Protects Retinal Photoreceptors: Evidence From a Rat Model of Light-Induced Photoreceptor Degeneration.

Brandli A; Johnstone DM; Stone J

Investigative ophthalmology & visual science.; Volume 57, Issue 13 2016/10/01

View Reference

Exercise reverses age-related vulnerability of the retina to injury by preventing complement-mediated synapse elimination via a BDNF-dependent pathway.

Chrysostomou V; Galic S; van Wijngaarden P; Trounce IA; Steinberg GR; Crowston JG

Aging cell.; Volume 15, Issue 6 2016/12/01

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The a2C-adrenoceptor antagonist, ORM-10921, has antipsychotic-like effects in social isolation reared rats and bolsters the response to haloperidol.

Uys M; Shahid M; Sallinen J; Dreyer W; Cockeran M; Harvey BH

Progress in neuro-psychopharmacology & biological psychiatry.; Volume 71, Issue 2016/11/03

View Reference

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