Human Interleukin 18 (IL-18) / IFN-gamma-inducing factor / IL-1 gamma ELISA Kit (2 plates)
Description
The human Interleukin 18 (IL-18) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-18 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-18 concentration are added to these wells and allowed to complex with the bound IL-18 antibody. A biotinylated anti-human IL-18 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-18 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-18 in samples such as sera, plasma and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.Labviva Id: LV-0358-0669
Specifications
| Attribute Type | Attribute Value |
| Species Reactivity | HUMAN |
| Antibody Isotype | Monoclonal-Polyclonal |
| Application Details | Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed. |
| Alternative Name | Interleukin-18; IL-18; IL18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; IGIF; IL1F4; |
| Target Protein | IL18 |
| References Count | 4.0000 |
| Uniprot Number Only | Q14116 |
| Uniprot Full Accession Number & Name | Q14116 IL18_HUMAN; |
| Storage | Store at 4 °C |
| Product Specificity | Human IL-18 |
| UNSPSC Code | 41116158 |
| Shipping Temperature | 2-8°C (on cold pack) |
| Expiry Date | This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C. |
| Applications | ELISA |
| Manufacturer | BIOSENSIS |
| Sensitivity | < 1 pg/mL |
| Immunogen | The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, Y33-D197 |
| Product Type | ELISA |
| Format | complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions., Ready to use Assay |
| Product Size | 2 plates |
| Kit Components | The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols. |
| Target Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Range | 15.6 pg/mL - 1000 pg/mL |
Protocols & Papers
Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells.
Fernandez MV; Miller E; Krammer F; Gopal R; Greenbaum BD; Bhardwaj N
Journal of leukocyte biology.; Volume 99, Issue 5 2016/05/01
Mutation in cytochrome b gene of mitochondrial DNA in a family with fibromyalgia is associated with NLRP3-inflammasome activation.
Cordero MD; Alcocer-Gómez E; Marín-Aguilar F; Rybkina T; Cotán D; Pérez-Pulido A; Alvarez-Suarez JM; Battino M; Sánchez-Alcazar JA; Carrión AM; Culic O; Navarro-Pando JM; Bullón P
Journal of medical genetics.; Volume 53, Issue 2 2016/02/01
AMPK Phosphorylation Modulates Pain by Activation of NLRP3 Inflammasome.
Bullón P; Alcocer-Gómez E; Carrión AM; Marín-Aguilar F; Garrido-Maraver J; Román-Malo L; Ruiz-Cabello J; Culic O; Ryffel B; Apetoh L; Ghiringhelli F; Battino M; Sánchez-Alcazar JA; Cordero MD
Antioxidants & redox signaling.; Volume 24, Issue 3 2016/01/20
NLRP3 inflammasome is activated in fibromyalgia: the effect of coenzyme Q10.
Cordero MD; Alcocer-Gómez E; Culic O; Carrión AM; de Miguel M; Díaz-Parrado E; Pérez-Villegas EM; Bullón P; Battino M; Sánchez-Alcazar JA
Antioxidants & redox signaling.; Volume 20, Issue 8 2014/03/10
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4642070, Thermo Scientific* Finnpipette* F2 Single Channel Pipetters, 10-100 µLPrice :328.97 -
4642110, Thermo Scientific* Finnpipette* F2 Single Channel Pipetters, 1000-10000 µLPrice :328.97