Human Interleukin 18 (IL-18) / IFN-gamma-inducing factor / IL-1 gamma ELISA Kit (1 plate)

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Description

Description

The human Interleukin 18 (IL-18) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-18 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-18 concentration are added to these wells and allowed to complex with the bound IL-18 antibody. A biotinylated anti-human IL-18 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-18 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-18 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.

Labviva Id: LV-0358-0679

Specifications

Specifications

More Information
Attribute Type Attribute Value
Species Reactivity HUMAN
Antibody Isotype Monoclonal-Polyclonal
Application Details Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Name Interleukin-18; IL-18; IL18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; IGIF; IL1F4;
Target Protein IL18
References Count 4.0000
Uniprot Number Only Q14116
Uniprot Full Accession Number & Name Q14116 IL18_HUMAN;
Storage Store at 4 °C
Product Specificity Human IL-18
UNSPSC Code 41116158
Shipping Temperature 2-8°C (on cold pack)
Expiry Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Applications ELISA
Manufacturer BIOSENSIS
Sensitivity < 1 pg/mL
Immunogen The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, Y33-D197
Product Type ELISA
Format complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions., Ready to use Assay
Product Size 1 plate
Kit Components The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Target Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Range 15.6 pg/mL - 1000 pg/mL

Supporting Literature

Protocols & Papers

Protocols & Papers

Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells.

Fernandez MV; Miller E; Krammer F; Gopal R; Greenbaum BD; Bhardwaj N

Journal of leukocyte biology.; Volume 99, Issue 5 2016/05/01

View Reference

Mutation in cytochrome b gene of mitochondrial DNA in a family with fibromyalgia is associated with NLRP3-inflammasome activation.

Cordero MD; Alcocer-Gómez E; Marín-Aguilar F; Rybkina T; Cotán D; Pérez-Pulido A; Alvarez-Suarez JM; Battino M; Sánchez-Alcazar JA; Carrión AM; Culic O; Navarro-Pando JM; Bullón P

Journal of medical genetics.; Volume 53, Issue 2 2016/02/01

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AMPK Phosphorylation Modulates Pain by Activation of NLRP3 Inflammasome.

Bullón P; Alcocer-Gómez E; Carrión AM; Marín-Aguilar F; Garrido-Maraver J; Román-Malo L; Ruiz-Cabello J; Culic O; Ryffel B; Apetoh L; Ghiringhelli F; Battino M; Sánchez-Alcazar JA; Cordero MD

Antioxidants & redox signaling.; Volume 24, Issue 3 2016/01/20

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NLRP3 inflammasome is activated in fibromyalgia: the effect of coenzyme Q10.

Cordero MD; Alcocer-Gómez E; Culic O; Carrión AM; de Miguel M; Díaz-Parrado E; Pérez-Villegas EM; Bullón P; Battino M; Sánchez-Alcazar JA

Antioxidants & redox signaling.; Volume 20, Issue 8 2014/03/10

View Reference

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