Mouse TNF-alpha ELISA Kit (1 plate)
DescriptionThe mouse TNF-alpha Kit is a sandwich ELISA. The capture antibody is an anti-mouse TNF-alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TNF-alpha concentration are added to these wells and allowed to complex with the bound TNF-alpha antibody. A biotinylated mouse TNF-alpha antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TNF-alpha in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Labviva Id: LV-0358-0468
|Attribute Type||Attribute Value|
|Application Details||Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.|
|Alternative Name||TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2;|
|Uniprot Number Only||P06804|
|Uniprot Full Accession Number & Name||P06804 TNFA_MOUSE;|
|Storage||Store at 4 °C|
|Product Specificity||Mouse TNF-alpha|
|Shipping Temperature||2-8°C (on cold pack)|
|Expiry Date||This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.|
|Sensitivity||< 1 pg/mL|
|Immunogen||The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids L80-L235|
|Format||complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions., Ready to use Assay|
|Product Size||1 plate|
|Kit Components||The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.|
|Target Cross Reactivity||Boster’s mouse TNF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNF alpha has been precoated onto 96-well plates. Standards (E.coli,L80-L235) and test samples are|
|Range||15.6 pg/mL - 1000 pg/mL(cellculturesupernates),7.8 pg/mL - 500 pg/mL(mouseserum,plasma)|
Protocols & Papers
Inhibiting tumor necrosis factor-a before amyloidosis prevents synaptic deficits in an Alzheimer's disease model.
Cavanagh C; Tse YC; Nguyen HB; Krantic S; Breitner JC; Quirion R; Wong TP
Neurobiology of aging.; Volume 47, Issue 2016/11/01
Expression of Phenotypic Astrocyte Marker Is Increased in a Transgenic Mouse Model of Alzheimer's Disease versus Age-Matched Controls: A Presymptomatic Stage Study.
Doméné A; Cavanagh C; Page G; Bodard S; Klein C; Delarasse C; Chalon S; Krantic S
International journal of Alzheimer's disease.; Volume 2016, Issue 2016
ßCTF-correlated burst of hippocampal TNFa occurs at a very early, pre-plaque stage in the TgCRND8 mouse model of Alzheimer's disease.
Cavanagh C; Colby-Milley J; Bouvier D; Farso M; Chabot JG; Quirion R; Krantic S
Journal of Alzheimer's disease : JAD.; Volume 36, Issue 2 2013
Contribution of brain inflammation to neuronal cell death in neuronopathic forms of Gaucher's disease.
Vitner EB; Farfel-Becker T; Eilam R; Biton I; Futerman AH
Brain : a journal of neurology.; Volume 135, Issue Pt 6 2012/06/01